Innovation
Clinical Research
Mechanisms of Action
Health Claims
Dosage
Formulations
Why choose Holixer^™?

Holixer^™ and it’s development holixer and it’s development

Holixer^™ and it’s development

People all over the world have two health concerns in common: high, chronic stress and its impact on healthy sleep. These issues are rising, unfortunately.

A longstanding Ayurvedic herb, Tulsi (also known as Holy Basil, Ocimum tenuiflorum) is commonly used as an adaptogen, which helps the body to physiologically attain healthful balance. When in the state of healthful balance, the body can more easily adapt to stressors, and this can set the stage for sleep improvement.

Holixer^™ is a unique Tulsi extract supported by human clinical research demonstrating value for stress management and reduction, improved sleep and higher quality of life. Beyond ashwagandha, tulsi is gaining tremendous interest from consumers dealing with hypersomnia, parasomnia, insomnia and various types of anxiety, such as social anxiety, and test/performance anxiety.

Tulsi extract is on par with ashwagandha for the management of stress, sleep quality, calm, good mood and overall quality of mental-emotional life.

Through innovative technology (patent-pending), Holixer^™ features the Holy Basil X factor. It is a low-dose solution for stress and sleep quality.

Traditional uses holixer Traditional uses

Traditional uses

Traditional Use of Tulsi/Holy Basil Tulsi has powerful significance in Ayurveda, where it is revered as “Mother Medicine of Nature” and “The Queen of Herbs,” as well as being referred to as “an elixir of life.” Throughout India, tulsi is used in lifestyle practices and spiritual rituals.

Ayurvedic physicians use tulsi for their patients to normalize kapha and vata - this supports its use in modern herbal supplementation as an adaptogen. The healing properties of tulsi have been researched in hundreds of in vitro, animal and human studies, all showing a range of benefits including adaptogenic and anti-stress capabilities. To date, there are 1,014 published research articles on tulsi.

Innovation

Natural Remedies’ research and development team worked diligently for five years to create Bioactive Optimization Technology (B.O.T^™), a systematic technique that preserves the multiple bioactive compounds in Holy Basil and optimizes their innate capabilities.

This bioactives-enhancement technology results in Ocimum-Bioactive Complex (OBC), providing our brand partners and their consumers with a measurable, distinctive Holy Basil herbal extract that is incomparable to all others.

Human Clinical Evidence of Holixer^™

Everyday Stress

Everyday Stress (Chronic Stress)

Perceived Stress Scale (PSS)
Hair Cortisol

Acute Stress

MAST (Maastricht Acute Stress Test)

Sleep

Athens Insomnia Scale (AIS)

Sleep Quality

Fitbit^®: Worn every night to bed

Sleep Efficiency

Quality of life (QoL)

Promis

Physical Function, Anxiety, Depression, Fatigue, Sleep disturbance & Social

Condition

Healthy participants with stress and sleep problems

Participants

100 volunteers (n=50 in each group)

Dose

250 mg/day

Duration

8 weeks

Evaluation - Stress:

Subjective: Perceived stress scale (PSS), Visual analogue scale (VAS) read more | Objective: Hair cortisol, Salivary cortisol, Salivary alpha amylase (sAA), Blood Pressure

Evaluation Sleep:

Subjective: Athens Insomnia Scale (AIS) Objective: Fitbit®

Evaluation QOL:

Subjective: PROMIS 29

Outcome

Holixer^™ demonstrated significantly greater improvements in stress and read more sleep scores compared to the placebo

Lopresti et al. (2022) Front. Nutr . 9:965130. doi:10.3389/fnut.2022.96513

Everyday stress: Perceived Stress Scale (PSS) - Subjective

Effect of Holixer^™ & Placebo on change in the Perceived stress scale (PSS)

Holixer^™

Placebo

2W

Holixer^™ start showing effect within two weeks

2X

Holixer^™ reduced the stress scores two times compared to placebo

Holixer^™ showed significant reduction in Perceived Stress Score with 38% VS placebo.

*Statistically-significant between-group difference in change in PSS scores over time (P = .009).

Everyday stress: Hair cortisol Objective

Effect of Holixer^™ & Placebo on change in Hair cortisol concentration

Holixer^™ showed statistically significant lower hair cortisol concentration compared to placebo.

! Statistically significant lower concentration in hair cortisol (p=0.03). After 8 weeks of intake !

Acute stress: Salivary cortisol- Objective

Effect of Holixer^™ & Placebo on Salivary cortisol

Placebo

Holixer^™

Benefits in acute stress was strongly correlated WIth reduction in salivary cortisol within 10 minutes (Holixer 113% Vs Placebo 163%).

Acute stress: Salivary alpha amylase (sAA) -Objective

Effect of Holixer^™ & Placebo on Salivary alpha amylase (sAA)

Placebo

Holixer^™

Benefits in acute stress was strongly correlated
with reduction in salivary alpha amylase
within 10 minutes.

Sleep: Athens Insomnia Scale (AIS) - Subjective

Effect of Holixer^™ & Placebo on sleep

Placebo

Holixer^™

Holixer^™ showed 50% improvement in sleep as compared to placebo.

*Statistically-significant (p<.025)

Sleep: Wearable Sleep tracker (Fitbit^®) - Objective

Effect of Holixer^™ & Placebo on sleep

Placebo

Holixer^™

Holixer^™ showed significant improvement in sleep efficiency from the baseline.

* Statistically significant from baseline

QoL-PROMIS 29 Scale- Subjective

Marked improvement in Fatigue, Anxiety & Sleep distrubance

Holixer^™ Mechanisms of Action

Health Claims*

The human clinical study on Holixer^™ allows for the following health claims:

USA
Europe
ROW

Helps maintain healthy cortisol levels during everyday stress

Regular intake helps the body quickly respond when facing acute stress

Helps you work relaxed during everyday stress

For resolving the irritability that ruins the day

Helps in physical and mental well-being during everyday stress

Day and night-time aid for everyday stress and occasional sleeplessness

Helps to be calm during everyday stress

Support resilience to combat everyday stressors

Helps improve sleep efficiency during occasional sleeplessness

Non-drowsy calm aid

Stay asleep longer during occasional sleeplessness naturally

Helps to maintain vitality*

Helps to maintain resistance to stress*

Helps maintain healthy cortisol levels during everyday stress

Regular intake helps the body quickly respond when facing acute stress

Helps you work relaxed during everyday stress

For resolving the irritability that ruins the day

Helps in physical and mental well-being during everyday stress

Day and night-time aid for everyday stress and occasional sleeplessness

Helps to be calm during everyday stress

Support resilience to combat everyday stressors

Helps improve sleep efficiency during occasional sleeplessness

Non-drowsy calm aid

Stay asleep longer during occasional sleeplessness naturally

Dosage:

Low dose 250 mg/day
(in single or two divided doses)

Holixer^™: Stands for the things that matter to you

Formulations possible with Holixer^™

Holixer^™: Sustainably making a difference at everystep

Holixer^™ has an attractive sustainability story that will meet consumers’ high standards. The raw material Holy Basil plants are grown and cultivated in accordance with Good Agricultural Practices, with natural nutrient-rich fertilizers and without the use of pesticides. The entire process allows for Natural Remedies to have end-to-end control over biomass.

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